Abstract. Type 2 diabetes (T2D) is a global pandemic with a strong genetic component, but most causal genes influencing the disease risk remain unknown. It is clear, however, that the pancreatic beta cell is central to T2D pathogenesis. In vitro gene-knockout (KO) models to study T2D risk genes have so far focused on rodent beta cells.
5/11/2016 · Electrophoretic mobility shift assay for NF-kB EndoC-?H2 cells were cultured at pH 7.4 (8 h) or 6.4 (1, 2 and 8 h) or with PMA (100 ng/ml at pH 7.4 for 8 h).
Loops were annotated using HiCCUPS at 5kB and 10kB resolutions with default Juicer parameters. This yielded a list of 9,100 loops in EndoC -ßH1 cells and 2,580 loops in Islet cells. EndoC -ßH1 ATAC-seq libraries were prepared as previously described (Varshney et al.
2017) and sequenced on an Illumina NextSeq 500 with 2 x 125 bp cycles.
Research. The EndoC ?H cell lines represent powerful tools to study functional human beta cells. We have produced 3 generations of the EndoC ?H cell line used by over 150 research groups across the world. Scharfmann, R.
Staels, W.
& Albagli, O. The supply chain of human pancreatic ? cell lines. J Clin Invest. 2019129 (9),3511–3520.
4/29/2020 · In vitro gene-knockout (KO) models to study T2D risk genes have so far focused on rodent beta cells. However, there are important structural and functional differences between rodent and human beta cell lines. With that in mind, we have developed a robust pipeline to create a stable CRISPR/Cas9 KO in an authentic human beta cell line ( EndoC -?H1).
Available Meshes for Importing into Playground. All the playground examples are loaded using the default environment and default camera methods.
5/22/2020 · Colli, M. L. et al. PDL1 is expressed in the islets of people with type 1 diabetes and is up-regulated by interferons-alpha and-gamma via IRF1 induction..
CD36 antibody treatment of the human B-cell line, EndoC -BH1, increased IRS1 and exocytotic protein levels, improved granule docking and enhanced insulin secretion. Our results demonstrate that B-cells from obese T2D donors have dysfunctional exocytosis likely due to an abnormal lipid handling represented by differential CD36 expression.
XIII Congress of the Iberian Association for Comparative Endocrinology. Bilbao, Spain, September 2023. The Iberian Association of Comparative Endocrinology (AIEC) was funded back in 1998. Our association is composed mainly by Spanish and Portuguese scientists that study different aspects of the vertebrate and invertebrate endocrinology.
Anthony is a firm believer in a patient-centered team approach. He has worked alongside Monica Joyce for many years: Monica Joyce, M.S.
R.D.
C.D.E. is a registered dietitian/nutritionist and a Certified Diabetes Educator. Monica has extensive experience in metabolic nutrition, intensive diabetes management (use of state of the art pumps and sensors) and weight regulation.
